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DsRed2 fluorescent protein

DsRed2 retains the benefits typical of red fluorescent proteins, such as a high signal-to-noise ratio and distinct spectral properties for use in multicolor labeling experiments. DsRed2 is a variant of Discosoma sp. red fluorescent protein (drFP583) (Matz et al. 1999) with faster maturation and lower non-specific aggregation. The DsRed2 sequence contains a series of silent base pair changes corresponding to human codon usage preferences for high expression in mammalian cells (Haas, Park, and Seed 1996). You may also be interested in DsRed-Express2, an alternative DsRed version with mutations that enhance its solubility, reduce its green emission, and accelerate its maturation.

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Cat. #	Product	Size	Price
632421	pDsRed2-Mito Vector	20 ug	USD $615.00
pDsRed2-Mito encodes a fusion of Discosoma sp. red fluorescent protein (DsRed2) and a mitochondrial targeting sequence of human cytochrome c oxidase subunit VIII (Mito). The targeting sequence is fused to the 5'-end of DsRed2, which is human codon-optimized for high expression in mammalian cells. pDsRed2-Mito is designed for fluorescent labeling of mitochondria. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back AcGFP1 is ideal for multicolor and fluorescence microscopy applications AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Back Organelles targeted by the subcellular localization vectors Organelles targeted by the subcellular localization vectors. Back Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants. Back 632421: pDsRed2-Mito Vector
632420	pIRES2-DsRed2 Vector	20 ug	USD $615.00
Bicistronic expression vector containing DsRed2 as a marker for transfection efficiency. A gene of interest is inserted into the MCS located upstream of the encephalomyocarditis virus (EMCV) internal ribosomal entry site (IRES). The IRES sequence allows both the gene of interest and DsRed2 to be translated simultaneously from the same mRNA transcript. DsRed2 is a human codon-optimized variant of wild-type DsRed that has been engineered for faster maturation and lower non-specific aggregation. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Map of the fluorescent pIRES2 bicistronic expression vectors Map of the fluorescent pIRES2 bicistronic expression vectors. Back Expression of two proteins from a single mRNA transcript Expression of two proteins from a single mRNA transcript. A fluorescent protein is translated from an internal ribosome entry site (IRES). Back 632420: pIRES2-DsRed2 Vector
632419	pDsRed2-Bid Vector	20 ug	USD $615.00
pDsRed2-Bid encodes a fusion of Discosoma sp. red fluorescent protein (DsRed2) and Bid, a member of the Bcl-2 family. In healthy, non-apoptotic cells, Bid resides in the cytosol as soluble protein. Upon induction of apoptosis, Bid translocates to mitochondria. In cells expressing the Bid-DsRed2 fusion, the translocation can be detected by fluorescence microscopy. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Monitoring Bid activation with the pDsRed2-Bid Vector Monitoring Bid activation with the pDsRed2-Bid Vector. 3T3 cells were transiently transfected with pDsRed2-Bid (Panels A & B). Approximately 24 hr later, cells were incubated with 700 nM staurosporine, an apoptosis inducing agent, at 37°C for 3 hr. Cells were then fixed and examined with a Zeiss Axioskop equipped with the appropriate light filters. In untreated cells (Panel A), the Bid reporter (Bid-DsRed2) distributes uniformly in the cytosol, as expected. Following induction (Panel B), the reporter translocates to the surface of mitochondria, forming intensely fluorescent clusters—clear evidence that the Bid pathway has been activated.
632418	pDsRed2-Peroxi Vector	20 ug	USD $615.00
pDsRed2-Peroxi encodes a fusion of Discosoma sp. red fluorescent protein (DsRed2) and the peroxisomal targeting signal 1 (PTS1). The PTS1 sequence is fused to the 3'-end of DsRed2 and encodes the tripeptide SKL, which targets the fusion protein to the matrix of peroxisomes. DsRed2 is human codon-optimized for high expression in mammalian cells. pDsRed2-Peroxi is designed for fluorescent labeling of peroxisomes. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back AcGFP1 is ideal for multicolor and fluorescence microscopy applications AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Back Organelles targeted by the subcellular localization vectors Organelles targeted by the subcellular localization vectors. Back Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.
632409	pDsRed2-ER Vector	20 ug	USD $615.00
pDsRed2-ER is a mammalian expression vector designed to label the endoplasmic reticulum in living cells. The vector encodes a fusion consisting of Discosoma sp. red fluorescent protein (DsRed2); the endoplasmic reticulum (ER) targeting sequence of calreticulin, fused to the 5' end of DsRed2; and the ER retention sequence, KDEL, fused to the 3' end of DsRed2. DsRed2 is a human codon-optimized variant of wild-type DsRed that has been engineered for faster maturation and lower non-specific aggregation. pDsRed2-ER can be introduced into mammalian cells using any standard transfection method. If required, stable transformants can be selected using G418. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back AcGFP1 is ideal for multicolor and fluorescence microscopy applications AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Back Organelles targeted by the subcellular localization vectors Organelles targeted by the subcellular localization vectors. Back Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants. Back 632409: pDsRed2-ER Vector
632408	pDsRed2-Nuc Vector	20 ug	USD $615.00
pDsRed2-Nuc encodes Discosoma sp. red fluorescent protein (DsRed2) fused with three copies of the nuclear localization signal (NLS) of the SV40 T-antigen. The NLS sequences are fused to the 3'-end of DsRed2. DsRed2 is a human codon-optimized variant of wild-type DsRed that has been engineered for faster maturation and lower non-specific aggregation. pDsRed2-Nuc is designed for fluorescent labeling of the nucleus in living cells. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back AcGFP1 is ideal for multicolor and fluorescence microscopy applications AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Back Organelles targeted by the subcellular localization vectors Organelles targeted by the subcellular localization vectors. Back Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.
632407	pDsRed2-C1 Vector	20 ug	USD $615.00
pDsRed2-C1 encodes DsRed2, a variant of the Discosoma sp. red fluorescent protein (DsRed) that has been engineered for faster maturation and lower, non-specific aggregation. DsRed2 contains six amino acid substitutions that accelerate chromophore formation and decrease protein aggregation. In addition, the coding sequence for DsRed2 is human codon-optimized for high expression in mammalian cells. pDsRed2-C1 allows genes cloned into the multiple cloning site (MCS) downstream of the DsRed2 coding sequence to be expressed as fusions to the C-terminus of DsRed2 in mammalian cells. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Schematic for fluorescent fusion protein vectors Schematic for fluorescent fusion protein vectors. Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector. Back Hygromycin DsRed-Monomer and AcGFP1 expression vectors Hygromycin DsRed-Monomer and AcGFP1 expression vectors. Vector maps of our DsRed-Monomer and pAcGFP1 Hyg-N1 (Panel A) and Hyg-C1 (Panel B) mammalian expression vectors for fusion applications. Each vector contains a hygromycin selection cassette. These vectors can be used together with our standard N1/C1 vectors containing the neomycin selection cassette to establish double stable cell lines expressing two different Living Colors proteins. Back 632407: pDsRed2-C1 Vector
632406	pDsRed2-N1 Vector	20 ug	USD $615.00
pDsRed2-N1 encodes DsRed2, a variant of Discosoma sp. red fluorescent protein, (DsRed). DsRed2 contains six amino acid substitutions that accelerate chromophore maturation and decrease protein aggregation. In addition, the coding sequence for DsRed2 is human codon-optimized for higher expression in mammalian cells. pDsRed2-N1 allows genes to be cloned into the multiple cloning site (MCS) upstream of the DsRed2 coding sequence to be expressed as fusions to the N-terminus of DsRed2. The unmodified vector will express DsRed2 in mammalian cells. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Schematic for fluorescent fusion protein vectors Schematic for fluorescent fusion protein vectors. Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector. Back Hygromycin DsRed-Monomer and AcGFP1 expression vectors Hygromycin DsRed-Monomer and AcGFP1 expression vectors. Vector maps of our DsRed-Monomer and pAcGFP1 Hyg-N1 (Panel A) and Hyg-C1 (Panel B) mammalian expression vectors for fusion applications. Each vector contains a hygromycin selection cassette. These vectors can be used together with our standard N1/C1 vectors containing the neomycin selection cassette to establish double stable cell lines expressing two different Living Colors proteins. Back Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1 Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1.
632405	pDsRed2-1 Vector	20 ug	USD $615.00
pDsRed2-1 encodes DsRed2, a variant of the Discosoma sp. red fluorescent protein (DsRed). DsRed2 contains six amino acid substitutions that accelerate chromophore maturation and decrease protein aggregation. In addition, the coding sequence for DsRed2 contains a series of silent base-pair changes for higher expression in mammalian cells. pDsRed2-1 can be used as a reporter to monitor the activity of promoters and promoter/enhancer combinations cloned into the multiple cloning site (MCS) upstream of the promoterless DsRed2 gene. Without the addition of a functional promoter, this vector will not express DsRed2. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Vector map for promoterless fluorescent protein vectors AcGFP1-1, DsRed2-1, DsRed-Express-1, HcRed1-1 and ZsGreen1-1 Vector map for promoterless fluorescent protein vectors pAcGFP1-1, pDsRed2-1, pDsRed-Express-1, pHcRed1-1 and pZsGreen1-1. Back Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1 Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1.
632404	pDsRed2 Vector	20 ug	USD $615.00
pDsRed2 encodes DsRed2, a variant of the Discosoma sp. red fluorescent protein (DsRed). DsRed2 contains six amino acid substitutions that accelerate chromophore maturation and decrease protein aggregation. In addition, the coding sequence for DsRed2 contains a series of silent base-pair changes for higher expression in mammalian cells. In pDsRed2, the DsRed2 coding sequence is flanked by two separate MCS regions at the 5' and 3' ends, making it possible to excise and shuttle the DsRed2 coding region into other expression vectors. pDsRed2 is intended to serve as a source of the DsRed2 cDNA; It can also be used in bacteria to produce DsRed2 protein. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Basic fluorescent protein vector map Basic fluorescent protein vector map. Use this bacterial expression vector as a source of the fluorescent protein gene. Note: There is a stop codon at the 5' end of the 3' MCS. The 3' MCS should not be used for cloning. Back Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1 Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1.

Overview

DsRed2 excitation and emission maxima are 563 and 582 nm, respectively
High solubility and low aggregation
DsRed-Express2 is an alternative DsRed version with mutations that enhance its solubility, reduce its green emission, and accelerate its maturation

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Applications

Monitoring transfection efficiency
Protein localization studies
Multicolor experiments with AcGFP1 or other fluorescent proteins

References

Haas, J., Park, E.-C. & Seed, B. Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr. Biol. 6, 315–324 (1996).

Matz, M. V et al. Fluorescent proteins from nonbioluminescent Anthozoa species. Nat. Biotechnol. 17, 969–973 (1999).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Lentiviral-mediated fluorescent protein transduction

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DsRed2 fluorescent protein

Notice to purchaser

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

Organelles targeted by the subcellular localization vectors

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

632421: pDsRed2-Mito Vector

Notice to purchaser

Map of the fluorescent pIRES2 bicistronic expression vectors

Expression of two proteins from a single mRNA transcript

632420: pIRES2-DsRed2 Vector

Notice to purchaser

Monitoring Bid activation with the pDsRed2-Bid Vector

Notice to purchaser

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

Organelles targeted by the subcellular localization vectors

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Notice to purchaser

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

Organelles targeted by the subcellular localization vectors

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

632409: pDsRed2-ER Vector

Notice to purchaser

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

Organelles targeted by the subcellular localization vectors

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Notice to purchaser

Schematic for fluorescent fusion protein vectors

Hygromycin DsRed-Monomer and AcGFP1 expression vectors

632407: pDsRed2-C1 Vector

Notice to purchaser

Schematic for fluorescent fusion protein vectors

Hygromycin DsRed-Monomer and AcGFP1 expression vectors

Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1

Notice to purchaser

Vector map for promoterless fluorescent protein vectors AcGFP1-1, DsRed2-1, DsRed-Express-1, HcRed1-1 and ZsGreen1-1

Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1

Notice to purchaser

Basic fluorescent protein vector map

Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1

Overview

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References

Additional product information