Magnetic-bead-based isolation of viral RNA/DNA and bacterial DNA from veterinary samples

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Product overview

Magnetic-bead-based isolation of viral RNA/DNA and bacterial DNA from veterinary samples

The NucleoMag VET kit is designed for the isolation of viral RNA/DNA and bacterial DNA from common veterinary samples such as whole blood, serum/plasma, feces, ear notches, or swabs using manual or automated processing. The procedure is based on the reversible adsorption of nucleic acids to paramagnetic beads. Sample lysis is achieved by incubation with a lysis buffer containing chaotropic ions and supplemented by Proteinase K digestion. Nucleic acids are bound to paramagnetic NucleoMag B-Beads in the presence of Binding Buffer VEB. After magnetic separation, the paramagnetic beads are subjected to successive wash steps to remove contaminants and salts, and highly pure RNA/DNA are eluted in low-salt Elution Buffer VEL or water.

One kit for common veterinary samples
High sensitivity, even with low viral titers
Robust, one-tube procedure minimizes risk of cross-contamination
Kit format allows for manual or automated processing on common liquid handling platforms

At-a-glance NucleoMag principle Procedure Example data

At-a-glance

	NucleoMag VET
Technology	Magnetic bead technology
Format	Highly reactive superparamagnetic beads
Processing	Manual or automated
Starting material*	<200 µl whole blood, serum, plasma <30 mg tissue (e.g., ear notches) <200 µl feces <200 µl swab wash solution
Fragment size	300 bp–approximately 50 kb
Elution volume	50–100 µl
Preparation time	45–120 min/96 preps (45 min for KingFisher)
Binding capacity	0.4 μg/μl beads

*For all sample types, start with a maxiumum of 200 µl liquid/homogenized sample.

NucleoMag principle

The NucleoMag family of products is based on magnetic bead technology. Nucleic acids are bound to the magnetic beads under chaotropic salt conditions, contaminants are washed away with ethanolic wash buffers, and pure RNA and DNA are eluted from the beads using elution buffer.

Procedure

Example data

Graph: detection of viral DNA from animal tissue samples

Graph: Detection of viral RNA and DNA from animal blood samples

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