Adherent human neural stem cells differentiated in RHB-A culture medium express neural lineage markers, including microtubule associate protein 2 (Panel B), neuron-specific class III beta-tubulin (Panel C), gamma amino butyric acid (Panel D), and glial fibrillary acidic protein (Panel E)

Adherent human neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express neural stem cell markers

Adherent mouse neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express sex determining region Y-box 2 (Sox2, red) and Nestin (green)

Adherent mouse neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express neural stem cell markers, including Nestin, Vimentin (3CB2), radial glial cell marker-2 (RC2), glial fibrillary acidic protein (GFAP), and microtubule associate protein (MAP)

Human neural cortex stem cell lines were differentiated into either neurons (MAP2, red) or astrocytes (GFAP, green) using RHB-A

Fold expansion profiles of human neural stem cell lines cultured by different vendor’s systems

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